Journal: Journal of visualized experiments : JoVE

Article Title: Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

doi: 10.3791/64457

Figure Lengend Snippet: Materials

Article Snippet: ​ Name Company Catalog Number Comments (3-Aminopropyl)triethoxysilane, 99% (APTES) Sigma Aldrich 440140-100ML 20 Gauge IT Series Angled Dispensing Tip Jensen Global JG-20-1.0-90 3/16" dia. x 1/16" thick Nickel Plated Magnet KJ Magnetics D31 3M (TC) 12X12-6-467MP DigiKey 3M9726-ND ACETONE ACS REAGENT ≥99.5% Signa Aldrich 179124-4L BD-20AC LABORATORY CORONA TREATER Electro-Technic Products 12051A Bovine Serum Albumin (BSA), Fraction V, 98%, Reagent Grade, Alfa Aesar VWR AAJ64100-09 Clear cast acrylic sheet McMaster-Carr 8560K181 Corning 100 mL Trypsin 10x, 2.5% Trypsin in HBSS [−] calcium, magnesium, phenol red, Porcine Parvovirus Tested VWR 45000-666 Countess II Automated Cell Counter Thermo Fisher Scientific AMQAX1000 CT-FIRE software LOCI - University of Wisconsin EGM-2 Endothelial Cell Growth Medium-2 BulletKit, (CC-3156 & CC-4176), Lonza CC-3162, 500 mL Lonza CC-3162 Glutaraldehyde 50% in aqueous solution, Reagent Grade, Packaging=HDPE Bottle, Size=100 mL VWR VWRV0875-100ML Graphtec CELITE-50 Graphtec CE LITE-50 HEPES (1 M) Thermo Fisher Scientific 15-630-080 High-Purity Silicone Rubber .010" Thick, 6" X 8" Sheet, 55A Durometer McMaster-Carr 87315K62 Human Umbilical Vein Endothelial cells Thermo Fisher Scientific C0035C Invitrogen Trypan Blue Stain (0.4%) Thermo Fisher Scientific T10282 Isopropanol Fisher Scientific A4154 Laser cutter Full Spectrum 20x12 H-series Microfluidics Syringe pump New Era Syringe Pumps NE-1002X Microman E Single Channel Pipettor, Gilson, Model M1000E Gilson FD10006 Molecular Probes Alexa Fluor 488 Phalloidin Thermo Fisher Scientific A12379 Molecular Probes Hoechst 33342, Trihydrochloride, Trihydrate Thermo Fisher Scientific H3570 Nutragen Bovine Atelo Collagen Advanced BioMatrix 5010-50ML Pbs (10x), pH 7.4 VWR 70011044.00 PBS pH 7.4 Thermo Fisher Scientific 10010049.00 Phosphate-buffered saline (PBS, 10x), with Triton X-100 Alfa Aesar J63521 Replacement carrier sheet for graphtec craft ROBO CC330L-20 USCUTTER GRPCARSHTN Restek Norm-Ject Plastic Syringe 1 mL Luer Slip Restek 22766.00 Silicon wafer University wafer 452 Sodium Hydroxide, ACS, Packaging=Poly Bottle, Size=500 g VWR BDH9292-500G Sylgard 184 VWR 102092-312 Thermo Scientific Pierce 20x PBS Tween 20 Thermo Fisher Scientific 28352.00 Open in a separate window Materials.

Techniques: Software, Staining, CRAfT Assay